multi exponential relaxation analysis mera toolbox Search Results


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multi gauge v3.0 software  (FUJIFILM)
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milliplex multi pathway magnetic bead 9 plex cell signaling multiplex assay kit  (Cell Signaling Technology Inc)
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elisa kit  (Danaher Inc)
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Danaher Inc elisa kit
Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
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multi exponential relaxation analysis mera toolbox  (MathWorks Inc)
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MathWorks Inc multi exponential relaxation analysis mera toolbox
Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
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multi-element calibration solution merk icp multi-element standard solution iv  (Merck KGaA)
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Merck KGaA multi-element calibration solution merk icp multi-element standard solution iv
Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
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multi-walled carbon nanotubes (mwcnts; of 10–40 in diameter and in length; purity: 93)  (CNT Co Ltd)
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CNT Co Ltd multi-walled carbon nanotubes (mwcnts; of 10–40 in diameter and in length; purity: 93)
Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
Multi Walled Carbon Nanotubes (Mwcnts; Of 10–40 In Diameter And In Length; Purity: 93), supplied by CNT Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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icp multi-element standard solution xxi certipur  (Merck KGaA)
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Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
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icp multi-element standard solution xvi  (Merck KGaA)
90
Merck KGaA icp multi-element standard solution xvi
Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion <t>molecules</t> <t>(MCP-1,</t> sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by <t>ELISA.</t> ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.
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Image Search Results


Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion molecules (MCP-1, sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by ELISA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.

Journal: The Journal of Biological Chemistry

Article Title: miR-147a targets ZEB2 to regulate ox-LDL-induced monocyte adherence to HUVECs, atherosclerotic plaque formation, and stability in atherosclerosis

doi: 10.1016/j.jbc.2023.104657

Figure Lengend Snippet: Ox-LDL injured HUVECs and promoted its adhesion to THP-1 cells and expressions of adhesion molecules. A , cell viability was detected by MTT assay in HUVECs treated with ox-LDL at different concentrations. B , LDH activity in HUVECs among groups. C , Ox-LDL promoted adhesion between HUVECs and THP-1 cells. D , quantification of adhesion conducted by counting the number of adherent THP-1 cells ( violet ) per field. E – G , the expressions of adhesion molecules (MCP-1, sICAM-1, and sVCAM-1) in supernatants of HUVECs after culture with ox-LDL were detected by ELISA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus 0. The data were expressed as mean ± SD. The one-way ANOVA was used to compare multiple groups, followed by post hoc Dunnett’s test. Experiments in this figure were repeated three times, and similar results were obtained. HUVEC, human umbilical vein endothelial cell; LDH, lactate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; Ox-LDL, oxidized low-density lipoprotein; sICAM-1, soluble intercellular cell adhesion molecule-1; sVCAM-1, soluble vascular cellular adhesion molecule-1.

Article Snippet: The operation steps were strictly carried out according to the instructions of the ELISA kit (human MCP-1 ELISA kit: ab179886, Abcam; human sICAM-1 ELISA kit: 70-EK189-96, MultiSciences; human sVCAM-1 ELISA kit: DVC00, R&D Systems).

Techniques: MTT Assay, Activity Assay, Enzyme-linked Immunosorbent Assay